[DOCKTESTERS] Fwd: [PAWG-TECH] Draft agenda for PCAWG-TECH teleconference

Miguel Vazquez miguel.vazquez at cnio.es
Wed Oct 5 15:56:19 EDT 2016


Yes Denis, it was my token. I uploaded by mistake and the revoked it.

On Oct 5, 2016 8:15 PM, "Denis Yuen" <Denis.Yuen at oicr.on.ca> wrote:

Hi,
FYI, I checked with the ICGC team, it doesn't look like a valid token, but
it might be an expired token.  If it is an expired token, it might be
useful to delete it from your token manager on the ICGC site to be doubly
sure.

------------------------------
*From:* docktesters-bounces+denis.yuen=oicr.on.ca at lists.icgc.org
[docktesters-bounces+denis.yuen=oicr.on.ca at lists.icgc.org] on behalf of
Denis Yuen [Denis.Yuen at oicr.on.ca]
*Sent:* October 5, 2016 11:43 AM
*To:* Miguel Vazquez; Francis Ouellette
*Cc:* docktesters at lists.icgc.org

*Subject:* Re: [DOCKTESTERS] Fwd: [PAWG-TECH] Draft agenda for PCAWG-TECH
teleconference

Hi,
Heads-up, is that token in https://github.com/mikisvaz/
PCAWG-Docker-Test/blob/master/icgc-storage-client-1.0.19/
conf/application.properties
<http://redir.aspx?REF=vLiQNVYrBBEiMoSd9Wt7fjPae2lu_9q7lzIrSaFlZIasbbhGS-3TCAFodHRwczovL2dpdGh1Yi5jb20vbWlraXN2YXovUENBV0ctRG9ja2VyLVRlc3QvYmxvYi9tYXN0ZXIvaWNnYy1zdG9yYWdlLWNsaWVudC0xLjAuMTkvY29uZi9hcHBsaWNhdGlvbi5wcm9wZXJ0aWVz>
a real token?

As for workflows, I would probably go in the order bwa-mem, delly, sanger
(which you have already done), dkfz

------------------------------
*From:* docktesters-bounces+denis.yuen=oicr.on.ca at lists.icgc.org
[docktesters-bounces+denis.yuen=oicr.on.ca at lists.icgc.org] on behalf of
Miguel Vazquez [miguel.vazquez at cnio.es]
*Sent:* October 5, 2016 4:44 AM
*To:* Francis Ouellette
*Cc:* kr2 at sanger.ac.uk; docktesters at lists.icgc.org
*Subject:* Re: [DOCKTESTERS] Fwd: [PAWG-TECH] Draft agenda for PCAWG-TECH
teleconference

Hello all,

I've created a repository in

https://github.com/mikisvaz/PCAWG-Docker-Test <http://UrlBlockedError.aspx>

With scripts that can help you run the tests. They can help download
samples, prepare the Dockerstore.json files and run them. I've added an
entry in the google doc.

I've ran the Sanger on the HCC1143 (Test) data and it seemed to have
completed correctly (I updated the table). I'm in the process now of
running the same test data in the DKFZ and sample DO50311 on the Delly
workflow.

I have two standing questions:

- What are the other workflows I need to test?
- What is the bedpe file used by DKFZ and how to produce it. Denis has
pointed me to some forum threads that I have to study a bit, but it seems
that we need to run something to produce it before we can run the workflow,
and I wonder how this fits into our testing scheme. I'll read more about it
when I have a chance.

Best regards



On Fri, Sep 30, 2016 at 3:52 PM, Francis Ouellette <francis at oicr.on.ca
<http://UrlBlockedError.aspx>> wrote:

> Dear Miguel and Junjun,
>
> Any more attempts on testing the PCAWG sanger docker container?
>
> If you reproduce the same error, we will need to involve Bryan and
> Keiran Raine <kr2 at sanger.ac.uk <http://UrlBlockedError.aspx>> (author of
> the container).
>
> Let’s get this one figured out.
>
> I am going to assume that the making of the docker container is what
> needs resolving.
>
> Brian: We may need your input here.
>
> Details of our current experiment should continue to be posted here:
>
> https://goo.gl/XX5BG9 <http://UrlBlockedError.aspx>
>
> Thank you all,
>
> francis
>
> PS would be good for others on list to follow directions on above google
> doc and also see if they can succeed on this workflow.
>
> Junjun and Miguel have tried different clouds, but used the sanger
> workflow, on the same data set.
>
> Thank you for trying to do this.
>
> Would be good if I heard back from anybody before Monday AM (tech call).
>
> @bffo
>
> PS I CCed Keiran, but waiting to hear back from Brian before we need to
> involve him some more.
> PPS Junjun/Miguel: maybe you can try the DKFZ docker as well? (on the same
> data set).
>
>
> --
> B.F. Francis Ouellette          http://oicr.on.ca/person/francis-ouellette
> <http://UrlBlockedError.aspx>
>
>
>
> Begin forwarded message:
>
> *From: *Christina Yung <Christina.Yung at oicr.on.ca
> <http://UrlBlockedError.aspx>>
> *Subject: **[PAWG-TECH] Draft agenda for PCAWG-TECH teleconference*
> *Date: *September 30, 2016 at 9:19:39 AM EDT
> *To: *"pawg-tech (pawg-tech at lists.icgc.org <http://UrlBlockedError.aspx>)"
> <pawg-tech at lists.icgc.org <http://UrlBlockedError.aspx>>
>
> Hi Everyone,
>
>
>
> Below is a draft agenda for Monday’s tech call.  Please let me know if you
> have any agenda items for discussion.
>
> https://wiki.oicr.on.ca/display/PANCANCER/2016-10-03+PCAWG-
> TECH+Teleconference <http://UrlBlockedError.aspx>
>
>
>
> Have a great weekend!
>
> Christina
>
>
> Call Info
>
> *Usual Time            9 AM Eastern Time, Mondays*
>
> *UK                          0208 322 2500*
>
> *Canada                  1-866-220-6419*
>
> *United States        **1-877-420-0272*
>
> *All Others              Please see attached PDF file
> <http://UrlBlockedError.aspx> with a list of numbers for other countries.*
>
> *Participant Code  5910819#*
> Agenda
>
>
>
> *Time*
>
> *Item*
>
> *Who*
>
> *Attachments/Links*
>
> 5min
>
> Welcome. Wait for group members to log on
>
> Christina Yung <http://UrlBlockedError.aspx>, OICR
>
> 10min
>
> Overall status
> Christina Yung <http://UrlBlockedError.aspx>, OICR
>
> ·         Linkouts to Most Current PCAWG Data
> <http://UrlBlockedError.aspx>
>
> ·         Report data issues to pcawg-data at icgc.org
> <http://UrlBlockedError.aspx>, GNOS issue to: Help at annaisystems.com
> <http://UrlBlockedError.aspx>
>
> ·         From Boston F2F: PCAWG datasets & dependencies
> <http://UrlBlockedError.aspx>
>
> *Action Items*
>
> 1.       [Joachim] Consensus SV - final?
>
> 2.       [Jakob] Consensus SNVs - changes to "SNV near indels" annotation?
>
> 3.       [Junjun] Specimen ID mapping for miRNA and methylation
>
> 4.       [Jonathan & Joachim] Consensus calls for cell lines, followed by
> filtering
>
> 5.       [Matthias & Gordon] Docker containers for filtering methods
>
> 6.       [Christina] Run alignment & variant workflows on medulloblastoma
> sample (tumor 40x, normal 30x) from ICGC benchmark to estimate false
> negative rate
>
> 7.       [Christina] Follow up with institutes interested in hosting
> PCAWG data long-term
>
> 8.       [All] As per Jennifer's email on Sept 16, please provide
> authorship information again or for the first time using PCAWG Author Form (
> http://goo.gl/forms/5Wq5x5X1DK <http://UrlBlockedError.aspx>). Save the
> link "Edit your response" so you can go back later to provide updates, for
> example about your evolving role in writing specific papers.
>
> 9.       [All] Contribute to the manuscripts on
>
> a.       infrastructure: https://docs.google.com/docume
> nt/d/10alAxrWLdLSyhci-rfNuVH13rFXCJkaY_rzf1KJn7nc/edit
> <http://UrlBlockedError.aspx>
>
> b.       variants: Paper ( https://goo.gl/g9CLsu
> <http://UrlBlockedError.aspx> ), Supplement ( https://goo.gl/EWYh7e
> <http://UrlBlockedError.aspx> )
>
> c.       Rogue's Gallery of Cancer Genome Sequencing Artifacts ( outline
> <http://UrlBlockedError.aspx> )
>
> 10.   [Junjun] Discuss PCAWG vs DCC glossary terms at next PCAWG-10/13
> conference
>
> 10min
>
> Status of dockerizing workflows
> Brian O'Connor <http://UrlBlockedError.aspx>, UCSC
>
> Gordon Saksena <http://UrlBlockedError.aspx>, Broad
>
> Francis Ouellette <http://UrlBlockedError.aspx>, OICR
> *Status of PCAWG Workflow ports to Dockstore*:
>
> Denis has been porting the Dockstore entries to CWL version 1.0 which is
> part of our effort to publish Dockstore (this doesn't affect the content of
> the pipelines, simply their "descriptors" which allow them to be runnable
> via Dockstore). Denis has also worked on testing BWA-Mem, Sanger, EMBL
> (which all work with CWL 1.0 and Kerian's test dataset) and has fixed
> issues with DKFZ and is testing the latter with a real sample shortly.
>
> 1.       BWA-Mem - Ready for testing by Francis' team
>
> 2.       Sanger - Ready for testing by Francis' team.
>
> 3.       EMBL - Ready for testing by Francis' team.
>
> 4.       DKFZ - Ready for testing by Francis' team. I've exchanged emails
> with Manuel Ballesteros who has been testing this pipeline.
>
> 5.       Broad - Variant calling (MuTect, dRanger, snowman), need some
> work, Gordan sent details previously
>
> 6.       OxoG - Waiting for Dimitri to provide OxoG docker
>
> 7.       Variantbam
>
> 8.       Consensus algorithm
>
> PCAWG Docker (Dockstore) Testing Working Group
> <http://UrlBlockedError.aspx>
>
> 5min
>
> Other business?
>
> Group
>
>
>
>
>
>
>
> *Christina K. Yung, PhD*
> Project Manager, Cancer Genome Collaboratory
>
> *Ontario Institute for Cancer Research*
> MaRS Centre
>
> 661 University Avenue, Suite 510
> Toronto, Ontario, Canada M5G 0A3
> Tel: 416-673-8578
>
> www.oicr.on.ca <http://UrlBlockedError.aspx>
>
>
>
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