[DOCKTESTERS] Fwd: [PAWG-TECH] Draft agenda for PCAWG-TECH teleconference
Keiran Raine
kr2 at sanger.ac.uk
Fri Oct 7 04:37:26 EDT 2016
Hi,
Can someone give me an idea what the errors you see on real data are for Sanger? Which real data is in use?
Also what is non-standard in the BWA-mem preprocessing, I didn't work on the actual workflow but I did give input at the beginning (and have a very simple to use version in house which can run from fastq, BAM or CRAM inputs).
Keiran Raine
Principal Bioinformatician
Cancer Genome Project
Wellcome Trust Sanger Institute
kr2 at sanger.ac.uk
Tel:+44 (0)1223 834244 Ext: 4983
Office: H104
> On 7 Oct 2016, at 07:27, Miguel Vazquez <miguel.vazquez at cnio.es> wrote:
>
> Hi all,
>
> The Delly workflow on DO50311 just finished correctly. I've added that to the table. It's important to point out that I consider a success just that the workflow completes, not that the results are what they need to be. That I guess we can do at a later stage.
>
> By the way, the Delly workflow just seems to produce SV, is that the case? no SNV? It also produces a bedpe file that I guess I can try on the DKFZ, which is what was missing. I'm going to try that now, albeit with no clear idea of what I'm doing I must say, I'm not familiar with any of this.
>
> Current status so far as I understand it is that only Delly works straigh out of the box on real data, but only produces only SV. Sanger works on test data and fails on real data. DKFZ fails on Test data and requires bedpe file for real data (produced by Delly?) BWA-Mem requires preprocesing the files?
>
> I have to say that from where I stand the preprocesing required by Delly and BWA-Mem is a bit inconvenient and makes the tools not stand-alone.
>
>
> I admit that I'm not an expert so I might have got some info wrong. Any comments on this please?
>
> Best
>
> Miguel
>
>
>
> On Fri, Sep 30, 2016 at 3:52 PM, Francis Ouellette <francis at oicr.on.ca <mailto:francis at oicr.on.ca>> wrote:
> Dear Miguel and Junjun,
>
> Any more attempts on testing the PCAWG sanger docker container?
>
> If you reproduce the same error, we will need to involve Bryan and
> Keiran Raine <kr2 at sanger.ac.uk <mailto:kr2 at sanger.ac.uk>> (author of the container).
>
> Let’s get this one figured out.
>
> I am going to assume that the making of the docker container is what
> needs resolving.
>
> Brian: We may need your input here.
>
> Details of our current experiment should continue to be posted here:
>
> https://goo.gl/XX5BG9 <https://goo.gl/XX5BG9>
>
> Thank you all,
>
> francis
>
> PS would be good for others on list to follow directions on above google
> doc and also see if they can succeed on this workflow.
>
> Junjun and Miguel have tried different clouds, but used the sanger
> workflow, on the same data set.
>
> Thank you for trying to do this.
>
> Would be good if I heard back from anybody before Monday AM (tech call).
>
> @bffo
>
> PS I CCed Keiran, but waiting to hear back from Brian before we need to involve him some more.
> PPS Junjun/Miguel: maybe you can try the DKFZ docker as well? (on the same data set).
>
>
> --
> B.F. Francis Ouellette http://oicr.on.ca/person/francis-ouellette <http://oicr.on.ca/person/francis-ouellette>
>
>
>
>> Begin forwarded message:
>>
>> From: Christina Yung <Christina.Yung at oicr.on.ca <mailto:Christina.Yung at oicr.on.ca>>
>> Subject: [PAWG-TECH] Draft agenda for PCAWG-TECH teleconference
>> Date: September 30, 2016 at 9:19:39 AM EDT
>> To: "pawg-tech (pawg-tech at lists.icgc.org <mailto:pawg-tech at lists.icgc.org>)" <pawg-tech at lists.icgc.org <mailto:pawg-tech at lists.icgc.org>>
>>
>> Hi Everyone,
>>
>>
>>
>> Below is a draft agenda for Monday’s tech call. Please let me know if you have any agenda items for discussion.
>>
>> https://wiki.oicr.on.ca/display/PANCANCER/2016-10-03+PCAWG-TECH+Teleconference <https://wiki.oicr.on.ca/display/PANCANCER/2016-10-03+PCAWG-TECH+Teleconference>
>>
>>
>> Have a great weekend!
>>
>> Christina
>>
>>
>>
>> Call Info
>> Usual Time 9 AM Eastern Time, Mondays
>> UK 0208 322 2500
>> Canada 1-866-220-6419
>> United States 1-877-420-0272
>> All Others Please see attached PDF file <https://wiki.oicr.on.ca/download/attachments/57776990/PAWG-TECH-Telecon-International-Numbers.pdf?version=1&modificationDate=1401456003000&api=v2> with a list of numbers for other countries.
>> Participant Code 5910819#
>> Agenda
>>
>> Time
>>
>> Item
>>
>> Who
>>
>> Attachments/Links
>>
>> 5min
>>
>>
>> Welcome. Wait for group members to log on
>>
>>
>> Christina Yung <https://wiki.oicr.on.ca/display/~cyung>, OICR
>>
>>
>>
>> 10min
>>
>>
>> Overall status
>>
>>
>>
>> Christina Yung <https://wiki.oicr.on.ca/display/~cyung>, OICR
>>
>>
>> ·
>> Linkouts to Most Current PCAWG Data <https://wiki.oicr.on.ca/display/PANCANCER/Linkouts+to+Most+Current+PCAWG+Data>
>>
>> ·
>> Report data issues to pcawg-data at icgc.org <mailto:pcawg-data at icgc.org>, GNOS issue to: Help at annaisystems.com <mailto:Help at annaisystems.com>
>>
>> ·
>> From Boston F2F:
>>
>> PCAWG datasets & dependencies <https://docs.google.com/spreadsheets/d/1VltMMLL5XDIFipM3zzLqZOGj5z-UxIMKLDbsuDiD2Q8/edit?usp=sharing>
>>
>> Action Items
>>
>> 1.
>> [Joachim] Consensus SV - final?
>>
>>
>> 2.
>> [Jakob] Consensus SNVs - changes to "SNV near indels" annotation?
>>
>>
>> 3.
>> [Junjun] Specimen ID mapping for miRNA and methylation
>>
>>
>> 4.
>> [Jonathan & Joachim] Consensus calls for cell lines, followed by filtering
>>
>>
>> 5.
>> [Matthias & Gordon] Docker containers for filtering methods
>>
>>
>> 6.
>> [Christina] Run alignment & variant workflows on medulloblastoma sample (tumor 40x, normal 30x) from ICGC benchmark to estimate false negative
>> rate
>>
>>
>> 7.
>> [Christina] Follow up with institutes interested in hosting PCAWG data long-term
>>
>>
>> 8.
>> [All] As per Jennifer's email on Sept 16, please provide authorship information again or for the first time using PCAWG Author Form (http://goo.gl/forms/5Wq5x5X1DK <http://goo.gl/forms/5Wq5x5X1DK>).
>> Save the link "Edit your response" so you can go back later to provide updates, for example about your evolving role in writing specific papers.
>>
>>
>> 9.
>> [All] Contribute to the manuscripts on
>>
>>
>> a.
>> infrastructure:
>>
>> https://docs.google.com/document/d/10alAxrWLdLSyhci-rfNuVH13rFXCJkaY_rzf1KJn7nc/edit <https://docs.google.com/document/d/10alAxrWLdLSyhci-rfNuVH13rFXCJkaY_rzf1KJn7nc/edit>
>>
>> b.
>> variants: Paper ( https://goo.gl/g9CLsu <https://goo.gl/g9CLsu> ), Supplement ( https://goo.gl/EWYh7e <https://goo.gl/EWYh7e> )
>>
>>
>> c.
>> Rogue's Gallery of Cancer Genome Sequencing Artifacts (
>>
>> outline <https://wiki.oicr.on.ca/download/attachments/66958609/Rogues%E2%80%99%20Gallery%20of%20Cancer%20Genome%20Sequencing%20Artefacts.pdf?version=1&modificationDate=1469802643000&api=v2> )
>>
>>
>> 10.
>> [Junjun] Discuss PCAWG vs DCC glossary terms at next PCAWG-10/13 conference
>>
>>
>> 10min
>>
>>
>> Status of dockerizing workflows
>>
>>
>>
>> Brian O'Connor <https://wiki.oicr.on.ca/display/~boconnor>, UCSC
>>
>> Gordon Saksena <https://wiki.oicr.on.ca/display/~gsaksena@broadinstitute.org>, Broad
>>
>> Francis Ouellette <https://wiki.oicr.on.ca/display/~francis>, OICR
>>
>>
>> Status of PCAWG Workflow ports to Dockstore:
>>
>> Denis has been porting the Dockstore entries to CWL version 1.0 which is part of our effort to publish Dockstore (this doesn't affect the content of the pipelines, simply their
>> "descriptors" which allow them to be runnable via Dockstore). Denis has also worked on testing BWA-Mem, Sanger, EMBL (which all work with CWL 1.0 and Kerian's test dataset) and has fixed issues with DKFZ and is testing the latter with a real sample shortly.
>>
>> 1.
>> BWA-Mem - Ready for testing by Francis' team
>>
>>
>> 2.
>> Sanger - Ready for testing by Francis' team.
>>
>>
>> 3.
>> EMBL - Ready for testing by Francis' team.
>>
>>
>> 4.
>> DKFZ - Ready for testing by Francis' team. I've exchanged emails with Manuel Ballesteros who has been testing this pipeline.
>>
>>
>> 5.
>> Broad - Variant calling (MuTect, dRanger, snowman), need some work, Gordan sent details previously
>>
>>
>> 6.
>> OxoG - Waiting for Dimitri to provide OxoG docker
>>
>>
>> 7.
>> Variantbam
>>
>>
>> 8.
>> Consensus algorithm
>>
>>
>> PCAWG Docker (Dockstore) Testing Working Group <https://wiki.oicr.on.ca/display/PANCANCER/PCAWG+Docker+%28Dockstore%29+Testing+Working+Group>
>> 5min
>>
>>
>> Other business?
>>
>>
>> Group
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> Christina K. Yung, PhD
>> Project Manager, Cancer Genome Collaboratory
>>
>> Ontario Institute for Cancer Research
>> MaRS Centre
>>
>> 661 University Avenue, Suite 510
>> Toronto, Ontario, Canada M5G 0A3
>> Tel: 416-673-8578
>>
>> www.oicr.on.ca <http://www.oicr.on.ca/>
>>
>> This message and any attachments may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this message in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this message may not be that of the organization.
>>
>>
>>
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>
>
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