[DOCKTESTERS] Questions about BiasFilter

Miguel Vazquez mikisvaz at gmail.com
Mon Feb 27 10:13:30 EST 2017


Dear Matthias,

I'm sorry that my sound was bad, it turns out I was using a Bluetooth
headset and was pacing away from my phone.

1) The first question I had regards the files hs37d5.fa and hs37d5.fa.fai
that are used by the docker file. On other workflows I was able to find
resource files such as these at the ICGC DCC

https://dcc.icgc.org/releases/PCAWG/reference_data/

Should these two file be placed there? right now I've configured it to use
the ones in the 1000 Genomes ftp

http://ftp.1000genomes.ebi.ac.uk/vol1/ftp/technical/reference/phase2_reference_assembly_sequence/

2) The other question is how should I validate the results. From what I
understood you suggest using the final merged VCF, correct?. I suppose I
can get it from GNOS using the information in ICGC, and I seem to remember
a field is present there indicating the filtering status.

Best regards

Miguel
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