[DOCKTESTERS] Thanks!
Keiran Raine
kr2 at sanger.ac.uk
Tue Mar 14 05:16:17 EDT 2017
Hi Junjun,
You won't be able to separate out the readgroups in the headers if the input is a merged BAM file. If there are different libraries, read lengths etc it will cause problems for insert-size determination (used in determining proper-pairs) and result in inter-library duplicate removal (by definition reads from different libraries can't be duplicates).
If you really need to do it this way you'd have to add a pre-processing step, bamtofastq can split a BAM into it's component readgroups in a single pass.
Regards,
Keiran Raine
Principal Bioinformatician
Cancer Genome Project
Wellcome Trust Sanger Institute
kr2 at sanger.ac.uk
Tel:+44 (0)1223 834244 Ext: 4983
Office: H104
> On 13 Mar 2017, at 21:16, Junjun Zhang <Junjun.Zhang at oicr.on.ca> wrote:
>
> Hi Keiran,
>
> Can you please comment on this, i.e., comparison between alignment done lane by lane v.s. done with all lanes mixed?
>
> Basically, we are trying to prepare input BAMs for testing PCAWG BWA MEM workflow. The starting point is the aligned BAM because we don't have the unaligned lane BAM any more. The key point here is: should input BAM organized by lanes, one lane one BAM? Or just one BAM containing all lanes?
>
> Thanks,
> Junjun
>
>
>
> From: Miguel Vazquez <mikisvaz at gmail.com <mailto:mikisvaz at gmail.com>>
> Date: Monday, March 13, 2017 at 2:31 PM
> To: Junjun Zhang <junjun.zhang at oicr.on.ca <mailto:junjun.zhang at oicr.on.ca>>
> Cc: George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>, Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
> Subject: Re: [DOCKTESTERS] Thanks!
>
>> Hi Junjun
>>
>> About the unaligned BAM files, in fact I do have them for the two test I've ran. I could put them available for George but I think he could just as well produce them on site, since he might have to do that anyway. But we can always explore that option, though right now I don't know of a simple way to move these files around.
>>
>> About the number of lanes let me just say good grief! This is the first time I hear about it. So if I understand you correctly I need to:
>>
>> 1- Download the metadata for the BAM file
>> 2- Determine the read_groups
>> 3- Split the BAM file according to these read_groups
>> 4- Unalign these BAM files and produce header files with different lanes
>> 5- Run BWA-Mem
>> 6- Compare collectively the reads from these BAM files with the original BAM
>>
>> Could you please confirm that this is the case? Is this consistent with the 3% mismatches? A similar percentage was found in the HCC1143, could this be the reason for that as well? Also I asked Keiran about these headers and he said there where OK. If you could please confirm that I need to do this extended process I'd be grateful, because its quite involved and there are concepts here I'm not familiar with.
>>
>> Regards
>>
>> Miguel
>>
>>
>> On Mon, Mar 13, 2017 at 6:51 PM, Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>> wrote:
>>> Hi Miguel,
>>>
>>> I thought you kept the unaligned sequence you prepared for the testing.
>>>
>>> Following your link about preparing unaligned input, I found this: https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/prepare_unaligned.sh#L16-L35 <https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/prepare_unaligned.sh#L16-L35>, which actually could explain the high mismatch rate.
>>>
>>> When BWA MEM workflow runs, the alignments are done one lane level BAM at a time, then merge the aligned BAM later: https://github.com/ICGC-TCGA-PanCancer/Seqware-BWA-Workflow/blob/develop/src/main/java/com/github/seqware/WorkflowClient.java#L201 <https://github.com/ICGC-TCGA-PanCancer/Seqware-BWA-Workflow/blob/develop/src/main/java/com/github/seqware/WorkflowClient.java#L201>
>>>
>>> I see the script prepare_unaligned.sh always generates one read group (i.e., lane) for normal or tumour, no matter how many read groups (lanes) in the aligned BAMs. This has big impact on the alignment result when lanes are aligned independently comparing aligned altogether.
>>>
>>> The PCAWG Sequence Submission SOP has a step to prepare unaligned BAM, but it only works when the input is single lane BAM file: https://wiki.oicr.on.ca/display/PANCANCER/PCAWG+%28a.k.a.+PCAP+or+PAWG%29+Sequence+Submission+SOP+-+v1.0#PCAWG(a.k.a.PCAPorPAWG)SequenceSubmissionSOP-v1.0-a)FollowthisifyoustartfromsinglelaneBAMfiles <https://wiki.oicr.on.ca/display/PANCANCER/PCAWG+%28a.k.a.+PCAP+or+PAWG%29+Sequence+Submission+SOP+-+v1.0#PCAWG(a.k.a.PCAPorPAWG)SequenceSubmissionSOP-v1.0-a)FollowthisifyoustartfromsinglelaneBAMfiles>
>>>
>>> So, I think in order to perform testing alignment workflow properly, we will need to prepare lane level unaligned BAM (one lane one BAM) as inputs. For example, this aligned BAM: https://gtrepo-ebi.annailabs.com/cghub/metadata/analysisFull/c9fa1c22-6432-4851-af67-30f4b4812c63 <https://gtrepo-ebi.annailabs.com/cghub/metadata/analysisFull/c9fa1c22-6432-4851-af67-30f4b4812c63>, it has 7 read groups (search for read_group). It needs to be converted to 7 individual lane level BAM files.
>>>
>>> Not sure whether it's the best way to do BAM splitting, but here is someone's Python code to do it: https://gist.github.com/seandavi/2014542 <https://gist.github.com/seandavi/2014542>
>>>
>>> Hope this helps,
>>> Junjun
>>>
>>>
>>>
>>> From: Miguel Vazquez <mikisvaz at gmail.com <mailto:mikisvaz at gmail.com>>
>>> Date: Monday, March 13, 2017 at 1:01 PM
>>> To: George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>
>>> Cc: Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>, Junjun Zhang <junjun.zhang at oicr.on.ca <mailto:junjun.zhang at oicr.on.ca>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>
>>>> Hi George,
>>>>
>>>> The analigned BAM files are not available as far as I know, rather you must unalign the final BAM files, the normal ones you get from ICGC or GNOS. This process is also in my scripts, as you see here:
>>>>
>>>> https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/run_batch.sh#L32 <https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/run_batch.sh#L32>
>>>>
>>>> About the steps in the workflows, I don't know them myself. I think you'll need to ask the developers, and not all workflows use the same underlying workflow enactment tool. Not an easy answer
>>>>
>>>>
>>>>
>>>> On Mon, Mar 13, 2017 at 5:57 PM, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>> wrote:
>>>>> Junjun told me this would provide value to the testing process, so I would like to kick off a test of the BWA_mem docker.
>>>>> Can somebody provide some quick instructions and the location of the unaligned BAM files that were used already?
>>>>>
>>>>> Also, do we have somewhere the steps involved in each workflow, so I can get an idea of how far they are while running?
>>>>> For example, s58_cgpPindel_pin2vcf_95 is three steps from finish, or 50 steps from finish…
>>>>>
>>>>> Thank you,
>>>>> George
>>>>>
>>>>> From: Miguel Vazquez <mikisvaz at gmail.com <mailto:mikisvaz at gmail.com>>
>>>>> Date: Monday, March 13, 2017 at 8:52 AM
>>>>>
>>>>> To: George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>
>>>>> Cc: Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>>, Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>>>
>>>>> Hi George,
>>>>>
>>>>> Answers inline
>>>>>
>>>>> On Mon, Mar 13, 2017 at 2:43 PM, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>> wrote:
>>>>>> Hi Miguel,
>>>>>>
>>>>>> I've started the test by running "bin/run_test.sh Sanger DO50398", so I guess with just one workflow running it should complete faster than two weeks.
>>>>>
>>>>> I think it still should take a long time. My scripts will run one workflow after another.
>>>>>
>>>>>>
>>>>>> Because I'm running in Collaboratory I've changed the "get_icgc_donor.sh" script to use a docker container that has the icgc client inside and pull data from Collaboratory. There is no "bam.bas" file downloaded, just a ".bam" and a ".bam.bai" files, not sure if this is an issue.
>>>>>>
>>>>>
>>>>> I wondered the same thing first time I did this, but this file is produced by the pipeline. There was some problem with this that was dealt with by the developers and updated in the docker. So I think you won't have a problem
>>>>>
>>>>>> By looking at the "bin/compare_result_type.sh" it looks like it's using the gnos client to pull down the existing VCF files for comparison reasons, but I think we store those files in Collaboratory as well, so I'll work with Junjun to adapt the script for this.
>>>>>>
>>>>>
>>>>> Let me know if you need any help
>>>>>
>>>>>> I think I initially tried to run the DKFZ workflow, but it complained about having to run Delly first, so I abandoned this for now.
>>>>>
>>>>> Yes, if you look at the run_batch.sh you will see that when using DKFZ it will always run Delly first. Delly prepares some files the the DKFZ file needs, namely related to copy number I believe.
>>>>>
>>>>>>
>>>>>> I'll set up a new VM and run the "run_batch.sh" on the DO52140 donor.
>>>>>
>>>>> Remember that you will need to add the relevant has-keys for the different files in the etc/donor_files.csv. Its a bit tedious right now. You need to go to the ICGC DCC and find these codes manually for the files you need. Ask me if you need help. Once you have all you can run all the workflows for that donor and evaluate results.
>>>>>
>>>>> https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/etc/donor_files.csv <https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/etc/donor_files.csv>
>>>>>
>>>>>
>>>>> Regards
>>>>>
>>>>> Miguel
>>>>>
>>>>>>
>>>>>> George
>>>>>>
>>>>>> From: Miguel Vazquez <mikisvaz at gmail.com <mailto:mikisvaz at gmail.com>>
>>>>>> Date: Monday, March 13, 2017 at 6:53 AM
>>>>>> To: George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>
>>>>>> Cc: Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>>, Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>>>>
>>>>>> Hi George,
>>>>>>
>>>>>> The Sanger workflow is very lengthy, it takes about two weeks in my tests.
>>>>>>
>>>>>> About correctness, my scripts also cover that part, if you are not using them they might still help you to clarify how we do it. The idea is to take each of the output files produced: SNV_MNV, Indel, SV, and CNV, for both germline and somatic and compare it with the result uploaded to GNOS (not all pipelines produce all files). This is the relevant part in the run_batch.sh script:
>>>>>>
>>>>>> https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/run_batch.sh#L42-L46 <https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/run_batch.sh#L42-L46>
>>>>>>
>>>>>> The bin/compare_result_type.sh script will take care of downloading the correct file from GNOS and running the comparison. The comparison itself is simple since all files are VCFs, it consists in taking out the variants in terms of chromosome, position, reference and alternative allele and measuring the overlaps.
>>>>>>
>>>>>> https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/compare_result_type.sh <https://github.com/mikisvaz/PCAWG-Docker-Test/blob/master/bin/compare_result_type.sh>
>>>>>>
>>>>>> About which donors to test, DO52140 is one Jonas and I have both tested and could be interesting to get a third opinion. Also, any other donor could be interesting to see if something new comes up. I'm not sure which options is best.
>>>>>>
>>>>>> Miguel
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Mon, Mar 13, 2017 at 5:12 AM, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>> wrote:
>>>>>>> Hi,
>>>>>>>
>>>>>>> I've started Sanger on DO50398 and it's been running for more than 24 hours, currently at "Workflow step succeeded: s58_bbAllele_merge_59"
>>>>>>>
>>>>>>> I just started a second run on a different VM on same donor, just to compare run times.
>>>>>>> The VM used has 8 cores, 48 GB of RAM and 1.1 TB disk and I'll send some monitoring graphs when it finishes the workflow, but I have no idea how to check its correctness.
>>>>>>>
>>>>>>> Give me a list of donors and what workflows you want me to run and I'll try to schedule them tomorrow.
>>>>>>>
>>>>>>> George
>>>>>>>
>>>>>>>
>>>>>>> From: Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>>
>>>>>>> Date: Sunday, March 12, 2017 at 10:45 PM
>>>>>>> To: Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>
>>>>>>> Cc: Miguel Vazquez <miguel.vazquez at cnio.es <mailto:miguel.vazquez at cnio.es>>, Denis Yuen <Denis.Yuen at oicr.on.ca <mailto:Denis.Yuen at oicr.on.ca>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>>>>>
>>>>>>> Thanks Miguel and Jonas for your help here!
>>>>>>>
>>>>>>> Do you have any update on the latest testing? Please feel free updating the wiki with any update: https://wiki.oicr.on.ca/display/PANCANCER/2017-03-13+PCAWG-TECH+Teleconference <https://wiki.oicr.on.ca/display/PANCANCER/2017-03-13+PCAWG-TECH+Teleconference>
>>>>>>>
>>>>>>> Regards,
>>>>>>> Junjun
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>> From: Jonas Demeulemeester <Jonas.Demeulemeester at crick.ac.uk <mailto:Jonas.Demeulemeester at crick.ac.uk>>
>>>>>>> Date: Saturday, March 11, 2017 at 7:15 PM
>>>>>>> To: George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>
>>>>>>> Cc: Miguel Vazquez <miguel.vazquez at cnio.es <mailto:miguel.vazquez at cnio.es>>, Junjun Zhang <junjun.zhang at oicr.on.ca <mailto:junjun.zhang at oicr.on.ca>>, Denis Yuen <Denis.Yuen at oicr.on.ca <mailto:Denis.Yuen at oicr.on.ca>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>>>>>
>>>>>>>> Hi George,
>>>>>>>>
>>>>>>>> Yup, I've been running the PCAWG dockers mainly using Miguel's set of scripts.
>>>>>>>> Give them a go and if you run into issues, just let us know!
>>>>>>>>
>>>>>>>> Cheers,
>>>>>>>> Jonas
>>>>>>>>
>>>>>>>>
>>>>>>>> On 11 Mar 2017, at 17:00, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>> wrote:
>>>>>>>>
>>>>>>>>> Sure, I'll give it a try and report later.
>>>>>>>>>
>>>>>>>>> Thank you,
>>>>>>>>> George Mihaiescu
>>>>>>>>> Senior Cloud Architect
>>>>>>>>>
>>>>>>>>> Ontario Institute for Cancer Research
>>>>>>>>> MaRS Centre
>>>>>>>>> 661 University Avenue
>>>>>>>>> Suite 510
>>>>>>>>> Toronto, Ontario
>>>>>>>>> Canada M5G 0A3
>>>>>>>>>
>>>>>>>>> Email: George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>
>>>>>>>>> Toll-free: 1-866-678-6427
>>>>>>>>> Twitter: @OICR_news
>>>>>>>>>
>>>>>>>>> www.oicr.on.ca <http://www.oicr.on.ca/>
>>>>>>>>>
>>>>>>>>> This message and any attachments may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this message in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this message may not be that of the organization.
>>>>>>>>>
>>>>>>>>>
>>>>>>>>>
>>>>>>>>> From: Miguel Vazquez <miguel.vazquez at cnio.es <mailto:miguel.vazquez at cnio.es>>
>>>>>>>>> Date: Saturday, March 11, 2017 at 10:57 AM
>>>>>>>>> To: Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>>
>>>>>>>>> Cc: Denis Yuen <Denis.Yuen at oicr.on.ca <mailto:Denis.Yuen at oicr.on.ca>>, Jonas Demeulemeester <jonas.demeulemeester at crick.ac.uk <mailto:jonas.demeulemeester at crick.ac.uk>>, George Mihaiescu <George.Mihaiescu at oicr.on.ca <mailto:George.Mihaiescu at oicr.on.ca>>, "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>>>>>> Subject: Re: [DOCKTESTERS] Thanks!
>>>>>>>>>
>>>>>>>>> Hi Junjun,
>>>>>>>>>
>>>>>>>>> I think Jonas has been using my scripts to run some of the tests, maybe George could try them as well, it should be very easy for him to try the Sanger, Delly+DKFZ, BWA-Mem, and the BiasFilter.
>>>>>>>>>
>>>>>>>>> https://github.com/mikisvaz/PCAWG-Docker-Test <https://github.com/mikisvaz/PCAWG-Docker-Test>
>>>>>>>>>
>>>>>>>>> He would just need to update the tokens for DACO access and the scripts will take care of downloading the BAM files, running the workflows and evaluating the result.
>>>>>>>>>
>>>>>>>>> The documentation there is reasonably updated, but if this sounds good then perhaps he could contact me and I could walk him through the details.
>>>>>>>>>
>>>>>>>>> Best regards
>>>>>>>>>
>>>>>>>>> Miguel
>>>>>>>>>
>>>>>>>>> On Fri, Mar 10, 2017 at 9:51 PM, Junjun Zhang <Junjun.Zhang at oicr.on.ca <mailto:Junjun.Zhang at oicr.on.ca>> wrote:
>>>>>>>>>> Dear Docktesters,
>>>>>>>>>>
>>>>>>>>>> George Mihaiescu, cloud architect, of the Collaboratory at OICR plans to run some bioinformatics workflows to test Collab environment.
>>>>>>>>>>
>>>>>>>>>> Just thought this is a good opportunity to use as extra help for testing out the PCAWG dockerized workflows.
>>>>>>>>>>
>>>>>>>>>> Miguel, Denis and others, what workflows / datasets do you think would be good for George to run?
>>>>>>>>>>
>>>>>>>>>> Thanks,
>>>>>>>>>> Junjun
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>> From: <docktesters-bounces+junjun.zhang=oicr.on.ca at lists.icgc.org <mailto:docktesters-bounces+junjun.zhang=oicr.on.ca at lists.icgc.org>> on behalf of Denis Yuen <Denis.Yuen at oicr.on.ca <mailto:Denis.Yuen at oicr.on.ca>>
>>>>>>>>>> Date: Wednesday, March 1, 2017 at 10:26 AM
>>>>>>>>>> To: "docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>" <docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>>
>>>>>>>>>> Subject: [DOCKTESTERS] Thanks!
>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>> Hi,
>>>>>>>>>>>
>>>>>>>>>>> Just wanted to say thanks to Miguel and Jonas for keeping the workflow testing data page up-to-date.
>>>>>>>>>>>
>>>>>>>>>>> https://wiki.oicr.on.ca/display/PANCANCER/Workflow+Testing+Data <https://wiki.oicr.on.ca/display/PANCANCER/Workflow+Testing+Data>
>>>>>>>>>>>
>>>>>>>>>>> As we work on new versions or debugging, it is invaluable to know what versions of the workflows have worked outside OICR, thanks!
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>> Denis Yuen
>>>>>>>>>>> Senior Software Developer
>>>>>>>>>>>
>>>>>>>>>>> OntarioInstituteforCancerResearch
>>>>>>>>>>> MaRSCentre
>>>>>>>>>>> 661 University Avenue
>>>>>>>>>>> Suite510
>>>>>>>>>>> Toronto, Ontario,Canada M5G0A3
>>>>>>>>>>> Toll-free: 1-866-678-6427
>>>>>>>>>>> Twitter: @OICR_news
>>>>>>>>>>> www.oicr.on.ca <http://www.oicr.on.ca/>
>>>>>>>>>>> This message and any attachments may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this message in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this message may not be that of the organization.
>>>>>>>>>>
>>>>>>>>>> _______________________________________________
>>>>>>>>>> docktesters mailing list
>>>>>>>>>> docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>
>>>>>>>>>> https://lists.icgc.org/mailman/listinfo/docktesters <https://lists.icgc.org/mailman/listinfo/docktesters>
>>>>>>>>>>
>>>>>>>>>
>>>>>>>> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT
>>>>>>>>
>>>>>>>
>>>>>>> _______________________________________________
>>>>>>> docktesters mailing list
>>>>>>> docktesters at lists.icgc.org <mailto:docktesters at lists.icgc.org>
>>>>>>> https://lists.icgc.org/mailman/listinfo/docktesters <https://lists.icgc.org/mailman/listinfo/docktesters>
>>>>>>>
>>>>>>
>>>>>
>>>>
>>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <https://lists.icgc.org/mailman/private/docktesters/attachments/20170314/428361f3/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: smime.p7s
Type: application/pkcs7-signature
Size: 2058 bytes
Desc: not available
URL: <https://lists.icgc.org/mailman/private/docktesters/attachments/20170314/428361f3/attachment-0001.bin>
More information about the docktesters
mailing list